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normal pituitary rna amplification microarray analysis  (Becton Dickinson)
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Becton Dickinson normal pituitary rna amplification microarray analysis
<t>Microarray</t> signal values and relative expression measured by real-time PCR for the genes FOS, GNAS, GADD45A, AKAP9, and NTS. Dotted lines indicate the expression of the corresponding genes in normal pituitaries. A, Studies performed in the MEN1-associated pituitary tumor, showing concordance with the expression pattern observed in microarray analysis, for all genes evaluated. B, Results from the sporadic pituitary adenoma. Except for GADD45A, the pattern of gene expression observed in microarray was confirmed by real-time PCR.
Normal Pituitary Rna Amplification Microarray Analysis, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Microarray signal values and relative expression measured by real-time PCR for the genes FOS, GNAS, GADD45A, AKAP9, and NTS. Dotted lines indicate the expression of the corresponding genes in normal pituitaries. A, Studies performed in the MEN1-associated pituitary tumor, showing concordance with the expression pattern observed in microarray analysis, for all genes evaluated. B, Results from the sporadic pituitary adenoma. Except for GADD45A, the pattern of gene expression observed in microarray was confirmed by real-time PCR.

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Unique Gene Expression Profile Associated with an Early-Onset Multiple Endocrine Neoplasia (MEN1)-Associated Pituitary Adenoma

doi: 10.1210/jc.2011-1127

Figure Lengend Snippet: Microarray signal values and relative expression measured by real-time PCR for the genes FOS, GNAS, GADD45A, AKAP9, and NTS. Dotted lines indicate the expression of the corresponding genes in normal pituitaries. A, Studies performed in the MEN1-associated pituitary tumor, showing concordance with the expression pattern observed in microarray analysis, for all genes evaluated. B, Results from the sporadic pituitary adenoma. Except for GADD45A, the pattern of gene expression observed in microarray was confirmed by real-time PCR.

Article Snippet: Normal pituitary RNA for amplification and microarray analysis was obtained commercially (BD Biosciences Clontech, Palo Alto, CA) and comprised a pool of poly A + RNA from 88 male/female (sudden death) Caucasians, ages 16–88 yr. Total tumor RNA (5 μg) and poly A + selected (0.2 μg) RNA from pooled normal pituitaries were subject to linear RNA amplification using commercial reagents (MessageAMP aRNA Kit; Ambion, Austin, TX) in a single round of amplification.

Techniques: Microarray, Expressing, Real-time Polymerase Chain Reaction